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pureblutm hoechst 33 342 nuclear staining dye  (Bio-Rad)


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    Bio-Rad pureblutm hoechst 33 342 nuclear staining dye
    Fig. 4 Compromised late endocytic trafficking of NDUFS3-silenced pancreatic cancer cell lines. (A-D) Relative protein abundance of RAB7, dynein, LAMP- 1, TFEB, ATP6V1G1, and RAB9 was assessed by Western blotting in YAPC and MIA PaCa-2 cells transfected with control RNA (Scr) or with two different NDUFS3 siRNAs (indicated as #1 and #2). The antibody against NDUFS3 was used to check the efficiency of RNA interference (RNAi). Quantification of differences was performed by densitometric analysis, normalizing against HSP90, and it was reported as the ratio of NDUFS3-silenced samples (#1 and #2) compared to their Scr control. (E) Late endocytic acid compartments were live stained in NDUFS3-silenced YAPC and MIA PaCa-2 cells (indicated as #1 and #2) and relative Scr controls using LysoTracker DND-99 dye (red). Nuclei were labeled with <t>PureBluTM</t> <t>Hoechst</t> 33,342 Nuclear Staining Dye (blue). White boxes indicate zoomed areas below. Scale bar: 10 μm. (F-K) Corrected total cell fluorescence (CTCF), number and size of acid compartments were determined by ImageJ software in NDUFS3-silenced YAPC and MIA PaCa-2 cells and reported as the ratio compared to their Scr control. Each measure was obtained by analyzing at least 50 cells/samples from three or more independent experiments. Values are the mean ± SEM of at least three independent experiments. *p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001
    Pureblutm Hoechst 33 342 Nuclear Staining Dye, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pureblutm+hoechst+33+342+nuclear+staining+dye/pm40369571-129-11-17?v=Bio-Rad
    Average 99 stars, based on 83 article reviews
    pureblutm hoechst 33 342 nuclear staining dye - by Bioz Stars, 2026-08
    99/100 stars

    Images

    1) Product Images from "Induced mitochondrial deficit by NDUFS3 transient silencing reduces RAB7 expression and causes lysosomal dysfunction in pancreatic cancer cells."

    Article Title: Induced mitochondrial deficit by NDUFS3 transient silencing reduces RAB7 expression and causes lysosomal dysfunction in pancreatic cancer cells.

    Journal: Cell communication and signaling : CCS

    doi: 10.1186/s12964-025-02214-y

    Fig. 4 Compromised late endocytic trafficking of NDUFS3-silenced pancreatic cancer cell lines. (A-D) Relative protein abundance of RAB7, dynein, LAMP- 1, TFEB, ATP6V1G1, and RAB9 was assessed by Western blotting in YAPC and MIA PaCa-2 cells transfected with control RNA (Scr) or with two different NDUFS3 siRNAs (indicated as #1 and #2). The antibody against NDUFS3 was used to check the efficiency of RNA interference (RNAi). Quantification of differences was performed by densitometric analysis, normalizing against HSP90, and it was reported as the ratio of NDUFS3-silenced samples (#1 and #2) compared to their Scr control. (E) Late endocytic acid compartments were live stained in NDUFS3-silenced YAPC and MIA PaCa-2 cells (indicated as #1 and #2) and relative Scr controls using LysoTracker DND-99 dye (red). Nuclei were labeled with PureBluTM Hoechst 33,342 Nuclear Staining Dye (blue). White boxes indicate zoomed areas below. Scale bar: 10 μm. (F-K) Corrected total cell fluorescence (CTCF), number and size of acid compartments were determined by ImageJ software in NDUFS3-silenced YAPC and MIA PaCa-2 cells and reported as the ratio compared to their Scr control. Each measure was obtained by analyzing at least 50 cells/samples from three or more independent experiments. Values are the mean ± SEM of at least three independent experiments. *p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001
    Figure Legend Snippet: Fig. 4 Compromised late endocytic trafficking of NDUFS3-silenced pancreatic cancer cell lines. (A-D) Relative protein abundance of RAB7, dynein, LAMP- 1, TFEB, ATP6V1G1, and RAB9 was assessed by Western blotting in YAPC and MIA PaCa-2 cells transfected with control RNA (Scr) or with two different NDUFS3 siRNAs (indicated as #1 and #2). The antibody against NDUFS3 was used to check the efficiency of RNA interference (RNAi). Quantification of differences was performed by densitometric analysis, normalizing against HSP90, and it was reported as the ratio of NDUFS3-silenced samples (#1 and #2) compared to their Scr control. (E) Late endocytic acid compartments were live stained in NDUFS3-silenced YAPC and MIA PaCa-2 cells (indicated as #1 and #2) and relative Scr controls using LysoTracker DND-99 dye (red). Nuclei were labeled with PureBluTM Hoechst 33,342 Nuclear Staining Dye (blue). White boxes indicate zoomed areas below. Scale bar: 10 μm. (F-K) Corrected total cell fluorescence (CTCF), number and size of acid compartments were determined by ImageJ software in NDUFS3-silenced YAPC and MIA PaCa-2 cells and reported as the ratio compared to their Scr control. Each measure was obtained by analyzing at least 50 cells/samples from three or more independent experiments. Values are the mean ± SEM of at least three independent experiments. *p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001

    Techniques Used: Quantitative Proteomics, Western Blot, Transfection, Control, Staining, Labeling, Fluorescence, Software



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    pureblutm hoechst 33 342 nuclear staining dye  (Bio-Rad)
    99
    Bio-Rad pureblutm hoechst 33 342 nuclear staining dye
    Fig. 4 Compromised late endocytic trafficking of NDUFS3-silenced pancreatic cancer cell lines. (A-D) Relative protein abundance of RAB7, dynein, LAMP- 1, TFEB, ATP6V1G1, and RAB9 was assessed by Western blotting in YAPC and MIA PaCa-2 cells transfected with control RNA (Scr) or with two different NDUFS3 siRNAs (indicated as #1 and #2). The antibody against NDUFS3 was used to check the efficiency of RNA interference (RNAi). Quantification of differences was performed by densitometric analysis, normalizing against HSP90, and it was reported as the ratio of NDUFS3-silenced samples (#1 and #2) compared to their Scr control. (E) Late endocytic acid compartments were live stained in NDUFS3-silenced YAPC and MIA PaCa-2 cells (indicated as #1 and #2) and relative Scr controls using LysoTracker DND-99 dye (red). Nuclei were labeled with <t>PureBluTM</t> <t>Hoechst</t> 33,342 Nuclear Staining Dye (blue). White boxes indicate zoomed areas below. Scale bar: 10 μm. (F-K) Corrected total cell fluorescence (CTCF), number and size of acid compartments were determined by ImageJ software in NDUFS3-silenced YAPC and MIA PaCa-2 cells and reported as the ratio compared to their Scr control. Each measure was obtained by analyzing at least 50 cells/samples from three or more independent experiments. Values are the mean ± SEM of at least three independent experiments. *p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001
    Pureblutm Hoechst 33 342 Nuclear Staining Dye, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pureblutm+hoechst+33+342+nuclear+staining+dye/pm40369571-129-11-17?v=Bio-Rad
    Average 99 stars, based on 1 article reviews
    pureblutm hoechst 33 342 nuclear staining dye - by Bioz Stars, 2026-08
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 4 Compromised late endocytic trafficking of NDUFS3-silenced pancreatic cancer cell lines. (A-D) Relative protein abundance of RAB7, dynein, LAMP- 1, TFEB, ATP6V1G1, and RAB9 was assessed by Western blotting in YAPC and MIA PaCa-2 cells transfected with control RNA (Scr) or with two different NDUFS3 siRNAs (indicated as #1 and #2). The antibody against NDUFS3 was used to check the efficiency of RNA interference (RNAi). Quantification of differences was performed by densitometric analysis, normalizing against HSP90, and it was reported as the ratio of NDUFS3-silenced samples (#1 and #2) compared to their Scr control. (E) Late endocytic acid compartments were live stained in NDUFS3-silenced YAPC and MIA PaCa-2 cells (indicated as #1 and #2) and relative Scr controls using LysoTracker DND-99 dye (red). Nuclei were labeled with PureBluTM Hoechst 33,342 Nuclear Staining Dye (blue). White boxes indicate zoomed areas below. Scale bar: 10 μm. (F-K) Corrected total cell fluorescence (CTCF), number and size of acid compartments were determined by ImageJ software in NDUFS3-silenced YAPC and MIA PaCa-2 cells and reported as the ratio compared to their Scr control. Each measure was obtained by analyzing at least 50 cells/samples from three or more independent experiments. Values are the mean ± SEM of at least three independent experiments. *p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001

    Journal: Cell communication and signaling : CCS

    Article Title: Induced mitochondrial deficit by NDUFS3 transient silencing reduces RAB7 expression and causes lysosomal dysfunction in pancreatic cancer cells.

    doi: 10.1186/s12964-025-02214-y

    Figure Lengend Snippet: Fig. 4 Compromised late endocytic trafficking of NDUFS3-silenced pancreatic cancer cell lines. (A-D) Relative protein abundance of RAB7, dynein, LAMP- 1, TFEB, ATP6V1G1, and RAB9 was assessed by Western blotting in YAPC and MIA PaCa-2 cells transfected with control RNA (Scr) or with two different NDUFS3 siRNAs (indicated as #1 and #2). The antibody against NDUFS3 was used to check the efficiency of RNA interference (RNAi). Quantification of differences was performed by densitometric analysis, normalizing against HSP90, and it was reported as the ratio of NDUFS3-silenced samples (#1 and #2) compared to their Scr control. (E) Late endocytic acid compartments were live stained in NDUFS3-silenced YAPC and MIA PaCa-2 cells (indicated as #1 and #2) and relative Scr controls using LysoTracker DND-99 dye (red). Nuclei were labeled with PureBluTM Hoechst 33,342 Nuclear Staining Dye (blue). White boxes indicate zoomed areas below. Scale bar: 10 μm. (F-K) Corrected total cell fluorescence (CTCF), number and size of acid compartments were determined by ImageJ software in NDUFS3-silenced YAPC and MIA PaCa-2 cells and reported as the ratio compared to their Scr control. Each measure was obtained by analyzing at least 50 cells/samples from three or more independent experiments. Values are the mean ± SEM of at least three independent experiments. *p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001

    Article Snippet: Before seeding, nuclei of cells were stained with 20 nM of PureBluTM Hoechst 33,342 Nuclear Staining Dye (Bio-Rad).

    Techniques: Quantitative Proteomics, Western Blot, Transfection, Control, Staining, Labeling, Fluorescence, Software